Journal: Materials Today Bio

Article Title: Hepatocyte growth factor in biosheets promotes autonomous regeneration of cutaneous tissue after transplantation onto a full-thickness skin defect

doi: 10.1016/j.mtbio.2026.102969

Figure Lengend Snippet: HGF is a key factor driving skin regeneration by biosheets (A-F) Administration of recombinant murine HGF in the skin regeneration model. (A) Schematic illustration of administering recombinant HGF in the mouse model of skin regeneration. (B) Macroscopic images of the wounds on day 14 post-wounding. Broken lines indicate the area of unclosed wounds. (C) The unclosed wound area on day 14 post-wounding. (D) Deeply stained areas (surrounded by yellow broken lines in (E)) on day 14 post-wounding. (E) Representative images of HE-stained dorsal skin tissues on day 14 post-wounding. Yellow broken lines indicate the deeply stained subcutaneous tissues. (F, G) High-magnification views of (E) HE staining and (F) vWF immunostaining. Arrowheads indicate vWF-positive capillary blood vessels. (H-M) In-situ neutralization of HGF in the skin regeneration model using anti-HGF antibody. (H) Schematic illustration of the experiment. (I) Macroscopic images of the HE-stained wounds on day 7. Black arrowheads indicate the regions corresponding to epithelial regeneration and yellow broken lines indicate the deeply stained subcutaneous tissues. (J, K) High-magnification views of (J) HE staining and (K) vWF immunostaining. Arrowheads indicate neovascular vessels. (L) The length of the regenerated epithelium on day 7 post-wounding as measured using histological images. (M) Deeply stained areas (surrounded by yellow broken lines in (I)) on day 7 post-wounding. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The sections were incubated overnight with rabbit anti-HGF (DF6326, 1:200, Affinity Bioscience, Cincinnati, OH, USA), rabbit anti-c-Met (718000, 1:50, Invitrogen, Waltham, MA, USA), and rabbit anti-phosphorylated Met (ab5662, 1:200, Abcam PLC, Cambridge, MA, USA) antibodies at 4 °C.

Techniques: Recombinant, Staining, Immunostaining, In Situ, Neutralization

Journal: STAR Protocols

Article Title: Protocol for isolating and assessing immunomodulatory ability of explant-cultured human amniotic membrane mesenchymal stem cells

doi: 10.1016/j.xpro.2025.104274

Figure Lengend Snippet:

Article Snippet: HGF β (D6S7D) XP Rabbit mAb (1:2000) , Cell Signaling Technology , Catalog No- 52445.

Techniques: Control, Membrane, Recombinant, Saline, Cell Culture, Knock-Out, Plasmid Preparation, Microscopy, cDNA Synthesis, Lysis, Software, Sterility, Transferring, Flow Cytometry, Inverted Microscopy, Spectrophotometry, Electrophoresis